restitution analysis

restitution analysis
Рестрикционное картирование
1. Техника конструирования рестрикционной карты конкретного участка ДНК, позволяющая определять сайты узнавания различных рестриктаз II типа в линейном масштабе: фрагменты анализируемой последовательности, или гена, вырезаются и электрофоретически (см. Электрофорез) разъединяются, затем их размеры сопоставляют и оценивают расстояние между ними на генетической карте.
2. Использование рестриктаз для определения соотношения экзонов и интронов в составе к.-л. гена, для чего применяют метод Берка-Шарпа (см. Берка-Шарпа метод) и др. Разрешающая способность метода — до 20 пар нуклеотидов.

Англо-русский толковый словарь генетических терминов. — М.: Изд-во ВНИРО. . 1995.

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